Omega2 metagenome assembly pipeline requires 3 steps:
Read merging using STORM
STORM is a tool for merging paired-end reads with variable insert sizes (i.e. for both overlapping and non-overlapping pairs). It calculates the read overlaps and calls the consensus. The advantage is that it can merge the non-overlapping reads with a third read connecting them. But it’s slower and needs more memory compare to k-mer method like BBMerge.
Error Correction using HERO
Metagenome Assembly using DISCO
DISCO is a multi threaded and multiprocess distributed memory overlap-layout-consensus (OLC) metagenome assembler. Disco was developed as a scalable assembler to assemble large metagenomes from billions of Illumina sequencing reads of complex microbial communities.